TheFluoroSpotassayisaninventivedevelopmentoftheELISpotassayandusesfluorescentdetectioninsteadofcolorimetric.Thisallowsforsimultaneousdetectionofseveralanalytes,whichisamajoradvantage.ThehumanIFN-γ/IL-2FluoroSpotkitisperfectforuserswhowishtohaveaconvenientassaywithminimizedassayvariABIlity.Itenablesenumerationofcellssecretingeitheroneorbothanalytesandincludeslowfluorescentplatespre-coatedwithmonoclonalcaptureantibodies,monoclonaldetectionantibodies,secondaryreagentsconjugatedtofluorophores,afluorescenceenhancerandananti-CD28monoclonalantibodyforco-stimulation.ApolyclonalactivatorforhumanTcells(mAbCD3-2)issuppliedasapositivecontrolforcytokinesecretion.
Pre-coatedplatesenableamorerapidassaywithminimizedvariability.
Foranalysis,werecommendtheuseofanautomatedFluoroSpotreaderequippedwithfiltersforFITC(excitation490nm/emission510nm)andCy3(excitation550nm/emission570nm).Filtersshouldhavehighspecificitytoavoidbleed-throughartifacts.Doublesecretingcellsareidentifiedbyacomputerizedoverlay.
AnalyteInformation
IFN-γ
Interferon-γ(IFN-γ,alsoknownasIFN-gamma,IFN-g,IFNgandInterferon-gamma)isproducedmainlybyactivatedTcellsandNKcells.Itisaproinflammatorycytokinethatactivatesmacrophagesandendothelialcells,butitalsoregulatesimmuneresponsesbyeffectingAPCandTandBcells.ProductionofIFN-γbyhelperTcellsaswellascytotoxicTcellsisahallmarkoftheTH1-typephenotype,thus,high-levelproductionofIFN-γistypicallyassociatedwitheffectivehostdefenseagainstintracellularpathogens.
IL-2
Interleukin-2(IL-2,IL2)isproducedexclusivelybyactivatedTcells.IL-2promotestheproliferation,differentiation,andsurvivalofantigen-activatedTcellsandNK-cells.
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Otherformats: | ClicktoviewallHumanIFN-γ,IL-2products |
WehaveusedMabtechELISPOTantibodiesandreagentsforseveralyears.Thehighqualityandstandardandcompetitivepricewillkeepushookedforyearstocome.RecentlywehavestartedworkingwithFluorospot,whichhasreallyhelpedusmoveforwardandhaveopenednewavenuesinourresearch.Asalwaysmanagementandtechnicalstaffhaveallprovidedimpeccableservice.
品牌介绍
Mabtech的主要检测目标包括:Amyloid-β、IgG2、apoA1、IgG2a、apoB、IgG2b、apoD、IgG2c、apoE、IgG3、apoH、IgG4、apoJ (Clusterin)、IgM、apoM、IL-1β、CD3、IL-2、CD4、IL-4、CD8、IL-5、CD28、IL-6、CD40、IL-8 (CXCL8)、CD43、IL-10、Galectin-3、IL-12 (p70)、GM-CSF、IL-12/-23 (p40)、Granzyme A、IL-13、Granzyme B、IL-17A、IFN-α2、IL-21、IFN-αpan、IL-22、IFN-γ、IL-23、Ig kappa、IL-29 (IFN-λ1)、Ig lambda、IL-31、IgA、Insulin、IgA1、Perforin、IgE、TGF-β1 (Latent TGF-β1)、IgEa、hioredoxin-1、IgG、TNF-α、IgG1
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