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当前位置: 首页 > 产品中心 > ELISPOT > Mabtech/人IFN-γ/IL-2荧光斑点试剂盒,预涂/FSP-0102-2/2板
商品详细Mabtech/人IFN-γ/IL-2荧光斑点试剂盒,预涂/FSP-0102-2/2板
Mabtech/人IFN-γ/IL-2荧光斑点试剂盒,预涂/FSP-0102-2/2板
Mabtech/人IFN-γ/IL-2荧光斑点试剂盒,预涂/FSP-0102-2/2板
商品编号: FSP-0102-2
品牌: Mabtech Limited
市场价: ¥15400.00
美元价: 9240.00
产地: 美国(厂家直采)
公司:
产品分类: ELISPOT试剂盒
公司分类: ELISPOT
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
FluoroSpotkitwithpre-coatedplatesforenumerationofcellssecretingIFN-γandIL-2

TheFluoroSpotassayisaninventivedevelopmentoftheELISpotassayandusesfluorescentdetectioninsteadofcolorimetric.Thisallowsforsimultaneousdetectionofseveralanalytes,whichisamajoradvantage.ThehumanIFN-γ/IL-2FluoroSpotkitisperfectforuserswhowishtohaveaconvenientassaywithminimizedassayvariABIlity.Itenablesenumerationofcellssecretingeitheroneorbothanalytesandincludeslowfluorescentplatespre-coatedwithmonoclonalcaptureantibodies,monoclonaldetectionantibodies,secondaryreagentsconjugatedtofluorophores,afluorescenceenhancerandananti-CD28monoclonalantibodyforco-stimulation.ApolyclonalactivatorforhumanTcells(mAbCD3-2)issuppliedasapositivecontrolforcytokinesecretion.

Pre-coatedplatesenableamorerapidassaywithminimizedvariability.

Foranalysis,werecommendtheuseofanautomatedFluoroSpotreaderequippedwithfiltersforFITC(excitation490nm/emission510nm)andCy3(excitation550nm/emission570nm).Filtersshouldhavehighspecificitytoavoidbleed-throughartifacts.Doublesecretingcellsareidentifiedbyacomputerizedoverlay.


AnalyteInformation

IFN-γ
Interferon-γ(IFN-γ,alsoknownasIFN-gamma,IFN-g,IFNgandInterferon-gamma)isproducedmainlybyactivatedTcellsandNKcells.Itisaproinflammatorycytokinethatactivatesmacrophagesandendothelialcells,butitalsoregulatesimmuneresponsesbyeffectingAPCandTandBcells.ProductionofIFN-γbyhelperTcellsaswellascytotoxicTcellsisahallmarkoftheTH1-typephenotype,thus,high-levelproductionofIFN-γistypicallyassociatedwitheffectivehostdefenseagainstintracellularpathogens.

IL-2
Interleukin-2(IL-2,IL2)isproducedexclusivelybyactivatedTcells.IL-2promotestheproliferation,differentiation,andsurvivalofantigen-activatedTcellsandNK-cells.

Application:
  • FluoroSpot
Reactivity:
  • Human
Contents:
  • FITC-conjugateddetectionmAb(7-B6-1)
  • BiotinylateddetectionmAb(MT8G10)
  • Anti-FITC-490
  • SA-550
  • anti-CD3mAb
  • Anti-CD28mAb(CD28-A)
  • Fluorescenceenhancer
  • 2Pre-coatedFluoroSpotplates(mAbs1-D1KandMT2A91/2C95)
Size:2plates
WehaveusedMabtechELISPOTantibodiesandreagentsforseveralyears.Thehighqualityandstandardandcompetitivepricewillkeepushookedforyearstocome.RecentlywehavestartedworkingwithFluorospot,whichhasreallyhelpedusmoveforwardandhaveopenednewavenuesinourresearch.Asalwaysmanagementandtechnicalstaffhaveallprovidedimpeccableservice.
品牌介绍
Mabtech的主要检测目标包括:Amyloid-β、IgG2、apoA1、IgG2a、apoB、IgG2b、apoD、IgG2c、apoE、IgG3、apoH、IgG4、apoJ (Clusterin)、IgM、apoM、IL-1β、CD3、IL-2、CD4、IL-4、CD8、IL-5、CD28、IL-6、CD40、IL-8 (CXCL8)、CD43、IL-10、Galectin-3、IL-12 (p70)、GM-CSF、IL-12/-23 (p40)、Granzyme A、IL-13、Granzyme B、IL-17A、IFN-α2、IL-21、IFN-αpan、IL-22、IFN-γ、IL-23、Ig kappa、IL-29 (IFN-λ1)、Ig lambda、IL-31、IgA、Insulin、IgA1、Perforin、IgE、TGF-β1 (Latent TGF-β1)、IgEa、hioredoxin-1、IgG、TNF-α、IgG1