IhavecomparedMabtechELISpotassaytoELISpotassayswerunatourcompanyonregularbasis"head-to-head"usingidenticalsamples.Mabtech"skitsprovedtobesuperiortoourprotocol,hadsignificantlylowerbackgroundandprovidedbetterandhigherdetectionofspots.
Thiskitisidealforuserswhowantaconvenientandsensitiveassay.TheassayisdesignedfortheenumerationofcellssecretinghumanTNF-α.ThekitincludesELISpotplatespre-coatedwithmonoclonalantibody,biotinylateddetectionantibody,Streptavidin-ALPandready-to-useBCIP/NBT-plussubstrate.
Pre-coatedplatesreduceassaytimeandminimizevariABIlity.
HumanTNF-αELISpotPLUSkitsarealsoavailablewithStreptavidin-HRPandTMBsubstrate.SeeRelatedproducts.
Speciescross-reactivity
Themonoclonalantibodiesinthiskitcross-reactwithTNF-αfromnon-humanprimates.SeetheHuman/NHPcross-reactivitytable.
AnalyteInformation
TNF-α
Tumornecrosisfactor-α(TNF-α,alsoknownasTNF-alpha,TNF-a,TNFa,andTumornecrosisfactor-alpha)isproducedbymanydifferentcelltypese.g.monocytes,macrophages,TcellsandBcells.AmongthemanyeffectsofTNF-α,areprotectionagainstbacterialinfection,cellgrowthmodulation,immunesystemregulationandinvolvementinsepticshock.
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Size: | 10plates |
Otherformats: | ClicktoviewallHuman/NHPTNF-αproducts |
品牌介绍
Mabtech的主要检测目标包括:Amyloid-β、IgG2、apoA1、IgG2a、apoB、IgG2b、apoD、IgG2c、apoE、IgG3、apoH、IgG4、apoJ (Clusterin)、IgM、apoM、IL-1β、CD3、IL-2、CD4、IL-4、CD8、IL-5、CD28、IL-6、CD40、IL-8 (CXCL8)、CD43、IL-10、Galectin-3、IL-12 (p70)、GM-CSF、IL-12/-23 (p40)、Granzyme A、IL-13、Granzyme B、IL-17A、IFN-α2、IL-21、IFN-αpan、IL-22、IFN-γ、IL-23、Ig kappa、IL-29 (IFN-λ1)、Ig lambda、IL-31、IgA、Insulin、IgA1、Perforin、IgE、TGF-β1 (Latent TGF-β1)、IgEa、hioredoxin-1、IgG、TNF-α、IgG1
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