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当前位置: 首页 > 产品中心 > ELISPOT > Mabtech/Human IL-1beta/IL-6/TNF-alpha FluoroSpot PLUS (FSP-121309-10)/FSP-121309-10
商品详细Mabtech/Human IL-1beta/IL-6/TNF-alpha FluoroSpot PLUS (FSP-121309-10)/FSP-121309-10
Mabtech/Human IL-1beta/IL-6/TNF-alpha FluoroSpot PLUS (FSP-121309-10)/FSP-121309-10
Mabtech/Human IL-1beta/IL-6/TNF-alpha FluoroSpot PLUS (FSP-121309-10)/FSP-121309-10
商品编号: FSP-121309-10
品牌: Mabtech Limited
市场价: ¥85800.00
美元价: 51480.00
产地: 美国(厂家直采)
公司:
产品分类: ELISPOT试剂盒
公司分类: ELISPOT
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
FluoroSpotkitforenumerationofcellssecretingIL-1β,IL-6andTNF-α

TheFluoroSpotassayisaninventivedevelopmentoftheELISpotassayandusesfluorescentdetectioninsteadofcolorimetric.Thisallowsforsimultaneousdetectionofseveralanalytes,whichisamajoradvantage.ThehumanIL-1β/IL-6/TNF-αFluoroSpotkitisperfectforuserswhowishtohaveaconvenientassaywithminimizedassayvariABIlity.Itenablesenumerationofcellssecretingone,twoorthreeanalytes.Thekitincludeslowfluorescentplatespre-coatedwithmonoclonalcaptureantibodies,monoclonaldetectionantibodies,secondaryreagentsconjugatedtofluorophoresandafluorescenceenhancer.LPSisincludedaspositivecontrolstimulus.

Theassayisrecommendedfortheanalysisofmacrophages,monocytesanddendriticcells.ThesekitshavenotbeenevaluatedforT-cells.

Foranalysis,werecommendtheuseofanautomatedFluoroSpotreaderequippedwithfiltersforexcitation490nm/emission510nm(LED490,FITC)for490-reagentdetection,excitation550nm/emission570nm(LED550,Cy3)for550-reagentdetectionandexcitation640nm/emission660nm(LED640,Cy5)for640-reagentdetection.Filtersshouldhavehighspecificitytoavoidbleed-throughartifacts.


AnalyteInformation

IL-1βInterleukin-1ß(IL-1ßorIL-1F2,alsoknownasInterleukin-1beta,IL-1beta,IL1bandInterleukin-1beta)isaproinflammatorycytokineandinducerofacutephaseresponses.IL-1ßisproducedprimarilybymonocytes,macrophages,anddendriticcellsafterinductionbymicrobes.

IL-6Interleukin6(IL-6,IL6)isapleiotropiccytokinethatisproducedbymanydifferentcelltypesandplaysaroleinawiderangeoffunctions,suchasimmuneresponses,acute-phasereactions,andhematopoiesis.AmongotherthingsitaugmentsantibodyproductionfromactivatedBcellsinvitro.

TNF-αTumornecrosisfactor-α(TNF-α,alsoknownasTNF-alpha,TNF-a,TNFa,andTumornecrosisfactor-alpha)isproducedbymanydifferentcelltypese.g.monocytes,macrophages,TcellsandBcells.AmongthemanyeffectsofTNF-α,areprotectionagainstbacterialinfection,cellgrowthmodulation,immunesystemregulationandinvolvementinsepticshock.

OrderingInformation

OrderstoMabtechcanbeplacedonline,byemail,orbyphone/faxtoanyofouroffices.

Email:Sendyourordertoorder@mabtech.com.

CustomersfromUSAorCanada:Pleasesendyourordertomabtech.usa@mabtech.com.
Application:
  • FluoroSpot
Reactivity:
  • Human
Format:FluoroSpotPLUS
Documentation:Datasheet/Protocol
Contents:
  • BAM-conjugateddetectionmAb(7P10)
  • BiotinylateddetectionmAb(39C3)
  • WASP-conjugateddetectionmAb(MT20D9)
  • Anti-BAM-490
  • SA-550
  • Anti-WASP-640
  • Positivecontrol(LPS)
  • Fluorescenceenhancer
  • 10Pre-coatedFluoroSpotplates(mAbs13A5,MT175andMT25C5)
Size:10plates
品牌介绍
Mabtech的主要检测目标包括:Amyloid-β、IgG2、apoA1、IgG2a、apoB、IgG2b、apoD、IgG2c、apoE、IgG3、apoH、IgG4、apoJ (Clusterin)、IgM、apoM、IL-1β、CD3、IL-2、CD4、IL-4、CD8、IL-5、CD28、IL-6、CD40、IL-8 (CXCL8)、CD43、IL-10、Galectin-3、IL-12 (p70)、GM-CSF、IL-12/-23 (p40)、Granzyme A、IL-13、Granzyme B、IL-17A、IFN-α2、IL-21、IFN-αpan、IL-22、IFN-γ、IL-23、Ig kappa、IL-29 (IFN-λ1)、Ig lambda、IL-31、IgA、Insulin、IgA1、Perforin、IgE、TGF-β1 (Latent TGF-β1)、IgEa、hioredoxin-1、IgG、TNF-α、IgG1